Study on the signaling pathways regulated by the biological activity of Xiaoyao San

Abstract

As a classic prescription for soothing the liver and strengthening the spleen, Xiaoyao San takes "harmonizing the liver and spleen" as the core, taking into account the dual regulation of emotion and physiology, and is a model of "holistic view" and "syndrome differentiation and treatment" in traditional Chinese medicine. Its modern research has revealed the scientific connotation of traditional prescriptions through multidisciplinary technology, providing an important reference for the treatment of complex chronic diseases. The network pharmacology system can analyze its "multi-component-multi-target-multi-pathway" action characteristics: pharmacological system analysis, and pharmacophysical system analysis can obtain: pharmacological system analysis. In this study, 8 herbs such as Bupleurum chinensis, Angelica sinensis, Licorice glabra and Paeonia alba were used as the object, and highly bioavailable active ingredients such as saikosaponin and peony glycosides were screened through TCMSP and other databases, which can be used to predict their targets and intersect with disease genes such as depression and irritable bowel syndrome, and obtain core regulatory targets such as SIRT1 and MAOA. Websites such as bioinformatics platforms can also be used to analyze their pathways. Network analysis showed that Xiaoyao San played a synergistic role in the pathology of liver depression and spleen deficiency by regulating neurotransmitters (5-HT, dopamine), inflammation (NF-B) and hormone metabolism (HIF-1, estrogen). The binding activity of key components to targets has been verified by molecular docking. This study reveals the molecular mechanism of "holistic regulation" of Xiaoyao San to provide a theoretical basis for the treatment of incurable diseases in traditional Chinese medicine, and also provides an example for the modern application of network pharmacology in traditional Chinese medicine.