The effect of active ingredients in Dark plum on uric acid metabolism pathway

Abstract

Hyperuricemia is a metabolic disease characterized by excessive uric acid levels in the blood due to purine metabolism disorders. It can lead to many complications, such as gout and kidney stones. The traditional Chinese medicine - Prunus mume has shown good therapeutic effects in clinical practice. Therefore, in this study, network pharmacology combined with molecular docking technology was adopted to systematically explore the related active components and their mechanisms of action of Prunus mume in the treatment of hyperuricemia. Method: From the perspective of network pharmacology, in this study, the active components of Prunus mume were screened through the TCMSP database, thereby obtaining 8 candidate components that conformed to the pharmacokinetic parameters (OB≥30%, DL≥0.18). In this study, target genes were standardized using the UniProt database to obtain 118 drug targets. A total of 1389 targets related to hyperuricemia were collected through the GeneCards and OMIM databases, and 39 common targets were obtained through Wayne analysis.This study demonstrated through GO and KEGG enrichment analysis that these targets were significantly enriched in biological processes such as inflammatory response, oxidative stress and purine metabolism, as well as related signaling pathways.From the perspective of molecular docking technology, the results of molecular docking indicate that the core active components (such as quercetin, kaempferol, etc.) have relatively strong binding capabilities with key targets (such as AKT1, TNF, etc.).Finally, this study revealed the potential mechanism of Prunus mume in treating hyperuricemia through the synergistic effect of "multiple components - multiple targets - multiple pathways", providing a new idea for the research on the modernization of traditional Chinese medicine.